The nucleotide sequence of nuclear U6 (4.7 S) RNA.

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The nucleotide sequence of nuclear U6 (4.7 S) RNA.

The low molecular weight RNA species which have been purified and characterized in this laboratory (T.S. Ro-Choi and H. Busch (1974) in The Cell Nucleus, Vol. 3, pp 151-208; Academic Press, New York) are now of interest because of their suggested role in processing of heterogeneous nuclear RNAs (Lerner, M.R., Boyle, J.A., Mount, S.M., Wolin, S.L., and Steitz, J.A. (1980) Nature, 283, 220-224). ...

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Nucleotide sequence of Physarum U6 small RNA

SEQUENCE ORIGIN: Small RNA molecule U6 was isolated from the total cellular RNA of vegetative Phvsarnm microplasmodia (1) by preparative polyacrylamide electroshoresis (2,3). The molecule was radiolabeled in vitro to high-specific-activity using [ T>)-pCp and RNA-ligase, and sequenced by direct chemical methods as we previously described (3). In this sequencing process, pseudouridine scores as ...

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Nucleotide sequence of wheat chloroplastid 4.5 S ribonucleic acid. Sequence homologies in 4.5 S RNA species.

The nucleotide sequence of wheat (Triticum aestivum L.) chloroplastid ribosome-associated 4.5 S RNA is U-A-A-G-G-U-G-A-G-C-G-G-C-G-A-G-A-C-G-A-G-C-C-G-U-U-U-A-A-A-U-A-G-G-U-G-U-C-A-A -G-U-G-G-A-A-G-U-G-C-A-G-U-G-A-U-G-U-A-U-G-C-A-G-C-U-G-A-G-G-C-A-U-C-C-U-A-A-C- G-A-A-C-G-A-A-C-G-A-U-U-U-G-A-A-COH. The sequence is highly conserved among chloroplastid 4.5 S RNAs but not related to either the 5 S...

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Transcription of a U6 Small Nuclear RNA Gene in Vitro

U6 small nuclear RNA (snRNA), an essential component of the eukaryotic spliceosomes, is unique in that it is ynthesized by RNA polymerase 111, while all other U-snRNAs are synthesized by RNA polymerase 11. U6 genes are notable for functional upstream regulatory elements which resemble RNA polymerase I1 regulatory sequence motifs. In this study, the optimal conditions for transcription of the U6...

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A small RNA of Mycoplasma capricolum that resembles eukaryotic U6 small nuclear RNA.

Mycoplasma capricolum, a parasitic prokaryote, contains several small stable RNAs, besides rRNAs and tRNAs. One of them, designated MCS4 RNA (125 nucleotides in length), has been isolated and sequenced. This RNA is abundant in the cell, and is encoded by two genes. Unexpectedly, MCS4 RNA has been found to reveal extensive sequence similarity to eukaryotic U6 snRNAs. This finding suggests that M...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1980

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)43587-9